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CryoEM - Revision history
2026-04-05T18:52:01Z
Revision history for this page on the wiki
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KevinYager at 13:11, 11 October 2017
2017-10-11T13:11:57Z
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<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 13:11, 11 October 2017</td>
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<tr><td class='diff-marker'>−</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div><del class="diffchange diffchange-inline">TBD</del></div></td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins class="diffchange diffchange-inline">'''Cryo-electron microscopy''' ('''CryoEM''') is transmission electron microscopy (TEM) performed at cryogenic temperatures. It is modernly frequently used as shorthand specifically for the reconstruction of the structure of a nanoscale objected via TEM measurements of frozen materials. For instance, to reconstruct the shape of a protein, many copies of the protein can be dispersed on a substrate and frozen (to cryogenic temperatures). TEM images can be acquired, which will contain (statistically) views of the protein from many different orientations. Using image analysis techniques, these protein images can be selected from the images, and an accurate 3D model of the protein can be reconstructed by registering all the individual projections together.</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div> </div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins class="diffchange diffchange-inline">CryoEM is advancing rapidly, and allowing nearly atomic resolution reconstruction of nanostructures (proteins, viruses, etc.) that would be difficult to measure using [[diffraction]]. In particular, conventional [[macromolecular crystallography]] (MX) requires one to create large, defect-free, single crystals of a target molecule. CryoEM, on the other hand, can be used on species that do not form ordered crystals.</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==See Also==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==See Also==</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>* Werner Kühlbrandt [http://science.sciencemag.org/content/343/6178/1443.full The Resolution Revolution] ''Science'' '''2014''', 343 (6178), 1443-1444. [http://dx.doir.org/10.1126/science.1251652 doi: 10.1126/science.1251652]</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>* Werner Kühlbrandt [http://science.sciencemag.org/content/343/6178/1443.full The Resolution Revolution] ''Science'' '''2014''', 343 (6178), 1443-1444. [http://dx.doir.org/10.1126/science.1251652 doi: 10.1126/science.1251652]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>* [http://journals.iucr.org/m/issues/2016/01/00/hi5639/index.html CryoEM at IUCrJ: a new era]</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>* [http://journals.iucr.org/m/issues/2016/01/00/hi5639/index.html CryoEM at IUCrJ: a new era]</div></td></tr>
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KevinYager
http://gisaxs.com/index.php?title=CryoEM&diff=5803&oldid=prev
KevinYager: /* See Also */
2017-10-11T13:07:21Z
<p><span dir="auto"><span class="autocomment">See Also</span></span></p>
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<td colspan="2" style="background-color: #fff; color: #222; text-align: center;">Revision as of 13:07, 11 October 2017</td>
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<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==See Also==</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>==See Also==</div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div><ins style="font-weight: bold; text-decoration: none;">* Werner Kühlbrandt [http://science.sciencemag.org/content/343/6178/1443.full The Resolution Revolution] ''Science'' '''2014''', 343 (6178), 1443-1444. [http://dx.doir.org/10.1126/science.1251652 doi: 10.1126/science.1251652]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>* [http://journals.iucr.org/m/issues/2016/01/00/hi5639/index.html CryoEM at IUCrJ: a new era]</div></td><td class='diff-marker'> </td><td style="background-color: #f8f9fa; color: #222; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>* [http://journals.iucr.org/m/issues/2016/01/00/hi5639/index.html CryoEM at IUCrJ: a new era]</div></td></tr>
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KevinYager
http://gisaxs.com/index.php?title=CryoEM&diff=5396&oldid=prev
KevinYager: Created page with "TBD ==See Also== * [http://journals.iucr.org/m/issues/2016/01/00/hi5639/index.html CryoEM at IUCrJ: a new era]"
2016-01-04T17:49:08Z
<p>Created page with "TBD ==See Also== * [http://journals.iucr.org/m/issues/2016/01/00/hi5639/index.html CryoEM at IUCrJ: a new era]"</p>
<p><b>New page</b></p><div>TBD<br />
<br />
==See Also==<br />
* [http://journals.iucr.org/m/issues/2016/01/00/hi5639/index.html CryoEM at IUCrJ: a new era]</div>
KevinYager