The classic, stereotypical, application of SAXS is to study the size and shape of particles dispersed in solution (including biological macromolecules). In these cases, one typically reduces the 2D area detector image into a 1D curve (I vs. q), where the slope of the curve (and/or oscillations within it) encode information about the structure. SAXS can also be used to measure ordered nanoscale systems (e.g. nanoscale superlattices), where one observes sharp peaks in the scattering data. A variety of new techniques have been developed specifically for measurement of ordered nanostructures (GISAXS, CD-SAXS, GTSAXS, etc.).
- Quantitative (with effort)
- Non-destructive (to a point)
- Rapid (depending on x-ray source; synchrotron vs. labscale)
- Small sample size possible (x-ray focusing)
- In-situ experiments possible
- Can vary sample conditions, and probe structural response
- Low information content: data fitting is an ill-posed problem, meaning that it is possible to be misled in data analysis.
- Complex data analysis.
- Complex/expensive instrumentation.
- Biological samples: proteins or other biomacromolecules in solution, macromolecule conformation, protein aggregation, virus assembly/clustering, etc.
- Soft matter: polymers, colloids
- Liquid crystals
- Lattices of nano-objects
- Nanocomposites, metals, minerals, etc.