Difference between revisions of "BioSAXS"

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'''BioSAXS''' refers to the use of small-angle [[x-ray]] [[scattering]] ([[SAXS]]) to probe biologically-relevant systems, such as proteins (or protein aggregates) in solution.
 
'''BioSAXS''' refers to the use of small-angle [[x-ray]] [[scattering]] ([[SAXS]]) to probe biologically-relevant systems, such as proteins (or protein aggregates) in solution.
 +
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Unlike [[crystallography]] or [[diffraction]] experiments, BioSAXS experiments typically do not generate distinct [[Bragg peaks]] or rings. Instead, a smooth decay of scattering intensity is observed at small scattering angles (which may look like [[diffuse scattering]]). However, with appropriate modeling, the specific form of this intensity decay can be fit in order to extract parameters of physical interest, including:
 +
* Radius of gyration, or molecular weight, of macromolecules (including proteins)
 +
* Particle shape (at low resolution)
 +
* Inter-particle distances
 +
* Aggregation or conformational state of macromolecules (degree of folding, denaturation, disorder)
  
 
==References==
 
==References==
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* Nielsen, S. S., Moller, M. & Gillilan, R. E. [http://journals.iucr.org/j/issues/2012/02/00/issconts.html High-throughput biological small-angle X-ray scattering with a robotically loaded capillary cell] ''J. Appl. Cryst.'' '''2012''', 45, 213-223. [http://dx.doi.org/10.1107/S0021889812000957 doi: 10.1107/S0021889812000957]
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* D. A. Jacques, J. M. Guss, D. I. Svergun and J. Trewhella [http://scripts.iucr.org/cgi-bin/paper?S0907444912012073 Publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution] ''Acta Cryst.'' '''2012''' D68, 620-626 [http://dx.doi.org/10.1107/S0907444912012073 doi: 10.1107/S0907444912012073]
 
*  Kevin N. Dyer, Michal Hammel, Robert P. Rambo, Susan E. Tsutakawa, Ivan Rodic, Scott Classen, John A. Tainer, Greg L. Hura  [http://link.springer.com/protocol/10.1007%2F978-1-62703-691-7_18 High-Throughput SAXS for the Characterization of Biomolecules in Solution: A Practical Approach] ''Methods in Molecular Biology'' '''2014''', 1091, 245-258  [http://dx.doi.org/10.1007/978-1-62703-691-7_18 10.1007/978-1-62703-691-7_18]
 
*  Kevin N. Dyer, Michal Hammel, Robert P. Rambo, Susan E. Tsutakawa, Ivan Rodic, Scott Classen, John A. Tainer, Greg L. Hura  [http://link.springer.com/protocol/10.1007%2F978-1-62703-691-7_18 High-Throughput SAXS for the Characterization of Biomolecules in Solution: A Practical Approach] ''Methods in Molecular Biology'' '''2014''', 1091, 245-258  [http://dx.doi.org/10.1007/978-1-62703-691-7_18 10.1007/978-1-62703-691-7_18]
 
*  Soren Skou, Richard E Gillilan & Nozomi Ando [http://www.nature.com/nprot/journal/v9/n7/full/nprot.2014.116.html Synchrotron-based small-angle X-ray scattering of proteins in solution] ''Nature Protocols'' '''2014''', 9, 1727-1739 [http://dx.doi.org/10.1038/nprot.2014.116 doi: 10.1038/nprot.2014.116]
 
*  Soren Skou, Richard E Gillilan & Nozomi Ando [http://www.nature.com/nprot/journal/v9/n7/full/nprot.2014.116.html Synchrotron-based small-angle X-ray scattering of proteins in solution] ''Nature Protocols'' '''2014''', 9, 1727-1739 [http://dx.doi.org/10.1038/nprot.2014.116 doi: 10.1038/nprot.2014.116]
 
* Lachlan W. Casey, Alan E. Mark and Bostjan Kobe  [http://www.publish.csiro.au/paper/CH14396.htm Small-Angle X-Ray Scattering for the Discerning Macromolecular Crystallographer] ''Australian Journal of Chemistry'' '''2014''', 67 (12) 1786-1792 [http://dx.doi.org/10.1071/CH14396 doi: 10.1071/CH14396]
 
* Lachlan W. Casey, Alan E. Mark and Bostjan Kobe  [http://www.publish.csiro.au/paper/CH14396.htm Small-Angle X-Ray Scattering for the Discerning Macromolecular Crystallographer] ''Australian Journal of Chemistry'' '''2014''', 67 (12) 1786-1792 [http://dx.doi.org/10.1071/CH14396 doi: 10.1071/CH14396]
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* D. A. Jacques, J. M. Guss, D. I. Svergun and J. Trewhella [http://scripts.iucr.org/cgi-bin/paper?S0907444912012073 Publication guidelines for structural modelling of small-angle scattering data from biomolecules in solution] ''Acta Cryst.'' '''2012''' D68, 620-626 [http://dx.doi.org/10.1107/S0907444912012073 doi: 10.1107/S0907444912012073]
 
 
* Bente Vestergaarda and Zehra Sayersb [http://journals.iucr.org/m/issues/2014/06/00/tj5006/index.html Investigating increasingly complex macromolecular systems with small-angle X-ray scattering] ''IUCrJ'' '''2014''', 1 (6), 523-529 [http://dx.doi.org/10.1107/S2052252514020843 doi: 10.1107/S2052252514020843]
 
* Bente Vestergaarda and Zehra Sayersb [http://journals.iucr.org/m/issues/2014/06/00/tj5006/index.html Investigating increasingly complex macromolecular systems with small-angle X-ray scattering] ''IUCrJ'' '''2014''', 1 (6), 523-529 [http://dx.doi.org/10.1107/S2052252514020843 doi: 10.1107/S2052252514020843]
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==See Also==
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* [http://www.macchess.cornell.edu/MacCHESS/biosaxs_whatis.html CHESS guide to BioSAXS]

Revision as of 18:51, 27 January 2015

BioSAXS refers to the use of small-angle x-ray scattering (SAXS) to probe biologically-relevant systems, such as proteins (or protein aggregates) in solution.

Unlike crystallography or diffraction experiments, BioSAXS experiments typically do not generate distinct Bragg peaks or rings. Instead, a smooth decay of scattering intensity is observed at small scattering angles (which may look like diffuse scattering). However, with appropriate modeling, the specific form of this intensity decay can be fit in order to extract parameters of physical interest, including:

  • Radius of gyration, or molecular weight, of macromolecules (including proteins)
  • Particle shape (at low resolution)
  • Inter-particle distances
  • Aggregation or conformational state of macromolecules (degree of folding, denaturation, disorder)

References

See Also

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